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After deparaffinization, sections were heated in an autoclave at 121 C for 5 min in 10 mM sodium citrate buffer (pH 6.0) or reacted with protease solution (Nichirei Biosciences, Tokyo, Japan) for epitope retrieval, after which endogenous peroxidase was inactivated by 0.3% hydrogen peroxide in methanol

Consequently, NADH levels were increased in the mutant cells (Fig

Since high levels of GSTs are expressed in the hepatocytes and it has previously been demonstrated that GSTs display sulfonamidase activity, three sulfonamide prodrugs of metformin were designed and synthesized 120

Variation in the fumonisin biosynthetic gene cluster in fumonisin-producing and nonproducing black aspergilli