glutathione sequence Treatments 101: Part 1 — The Mother of All Antioxidants Glutathione
Description
doi: 10.1042/BSR20171440
ONE OF EVERYTHING 73.00 When you need EVERYTHING, weve got you covered
To determine the contribution of stress-induced gene reprograming, we took advantage of public RNA-seq results from the Gene Expression Omnibus for MCF7 cells in stress response
Samples were alkylated with 20 mM iodoacetamide (IOA) and incubated at room temperature for 1 h in the dark before the reaction was quenched with 2.5 mM dithiothreitol (DTT) and diluted to 0.1% SDC in 50 mM triethylammonium bicarbonate (TEAB) prior to proteolytic digest
