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It should be noted that the BBB is a complex interface composed not only by endothelial cells but also by astrocytes and perycites 23, 31 , and these cell types may also influence BBB transport of (poly)phenol metabolites in vivo

[DOI] [PubMed] [Google Scholar] 193.Beard J.L., et al

2.6.7 Transwell migration and invasion assay Cells were pretreated with 0 or 50 nM 4-EA for 48 h before seeding into the chamber

The assay design incorporates a control tube to prevent thiol interference with GPx enzyme activity