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If you're working with a lyophilized (freeze-dried) powder, proper reconstitution is critical

To assess the targeting of cPLT, DiO-labeled aPLT or DiO-labeled cPLT were added to the LPS-stimulated HUVECs and incubated at 37 C for 2 h

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Release of GABA, thus leads to inhibition of the optic nerve
