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Use of PDE inhibitors delays meiosis reinitiation and slows cumulus expansion kinetics, which prolongs maintenance of the gap bonds between the oocyte and cumulus cells 120
The beads were washed by buffer A 1 and the bound EBOV polymerase complexes were eluted by buffer B 1 containing 25 mM HEPES, pH 7.5, 300 mM NaCl, 1 mM TCEP supplemented with 2.5 mM desthiobiotin
The cells were washed, fixed and then analyzed with the use of a FACSCalibur instrument
Molecular Pharmacology, 8 , 20322038
