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Description
The method typically achieves greater than 90% purity of the GST-tagged protein from crude lysates

However, both the baseline permeability and permeability after the addition of gliadin were higher and the luminal zonulin release was more pronounced and prolonged in patients with CeD

Kusunose N, Koyanagi S, Hamamura K, Matsunaga N, Yoshida M, Uchida T, Tsuda M, Inoue K, Ohdo S (2010) Molecular basis for the dosing time-dependency of anti-allodynic effects of gabapentin in a mouse model of neuropathic pain

This decline has systemic consequences
