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Esposito BP, Breuer W, Sirankapracha P, Pootrakul P, Hershko C, Cabantchik ZI

Simultaneously, some cells undergo cell cycle arrest and become senescent owing to the inhibition of cyclin-dependent kinases mediated by p21 activation through the DDR pathway 11

The extracellular concentration of glutamine is around 0.7 mM, the most abundant extracellular amino acid in vivo, while the most abundant intracellular amino acid is glutamate, depending on the type of cell ranging between 2 and 20 mM [3]

In some cases, both the N- and C- termini may require tagging in case one terminus has the potential to disrupt binding interaction or protein function