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Reaction steps: 95 C for 15 min, 40 cycles of 95 C 15 s, 60 C 30 s, and 72 C 10 s
For example, insulin/insulin-like growth factor (IGF) signaling (IIS), which affects aging in many model organisms, integrates nutritional cues, and regulates fat storage in many animals (Fontana et al., 2010b)
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