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If no, go to step ii
Divide the total amount in the vial by the total mL of diluent added to get concentration, then calculate how much volume is needed for your dose
Some sources point to reduced oxygenation of the follicle when B12 is deficient
An affinity purified F(ab) 2 rabbit anti-human IgG (H+L) conjugated to Alexa Fluor 488 was obtained from Jackson ImmunoResearch (West Grove, PA, USA)
