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Sanger sequencing was done using routine protocols on an ABI3730 sequencing machine (Applied Biosystems, Foster City, USA)
Sections were washed with PBS, and stained with Alexa Fluor 488, 594, or 647 conjugated secondary antibodies in PBS, for 2 h at RT
Contrary to the long-lasting belief that IBS is not associated with organic changes, there is now growing evidence supporting that structural, although subtle, changes may be found in the gastrointestinal tract of large subgroups of these patients (197)
It works like a graphics tablet perfect for drawing, making notes, or editing in apps like Photoshop
