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Perhaps one of the most debated topics between peptide users and skeptics

Blocking was followed by incubation with rabbit monoclonal anti-FOS primary antibody (1:1,000 dilution, Cell Signalling 2250S) or rabbit polyclonal anti-Fluoro-Gold primary antibody (1:1000 Fluorochrome) in the same blocking solution overnight for 16 h and then with Alexa Fluor 594-conjugated donkey anti-rabbit IgG secondary fluorescent antibody (1:500 dilution, Invitrogen A21207) for 2 h at room temperature

NIDDKD, 2021)
