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The mixture was kept in a boiling water bath for 20 min, cooled with ice-cold water at 4 C, and then centrifuged at 3500 rpm for 10 min

Abstract Ferroptosis is a recently recognized form of non-apoptotic regulated cell death and usually driven by iron-dependent lipid peroxidation and has arisen to play a significant role in cancer biology

10.3390/ijerph19148312 340

Lactate shuttles in nature
