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Galloway and McLellan, 1998

In brief, 12.5 g mitochondrial protein was incubated with Tris-HCl (pH 8.1), 0.2 mM NaN 3 , 1 M antimycin A, 0.1% fat-free BSA, 50 M CoQ1 and 64 M DCPIP at 30C for 5 min

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The supraphysiologic level of hepatic SAMe level in the Gnmt KO mice resulted in hypermethylation of the promoter regions of Ras and Janus kinase (JAK)/signal transducer and activator of transcription (STAT) inhibitors (Ras-association domain family/tumor suppressor (RASSF) 1 and 4 and suppressor of cytokine signaling (SOCS) 13 and cytokine-inducible SH2-protein), resulting in decreased expression of these inhibitors and increased expression and activity of Ras and JAK/STAT pathways [37]