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Network pharmacology can be employed to identify related pathways explaining the operational mechanism of Rg1, functioning as an effective tool

10.1016/j.pneurobio.2019.04.001 [DOI] [PubMed] [Google Scholar] Sochocka M., Diniz B

The primary hepatocytes were initially cultured in DMEM medium for 24 hours, after which the wells were washed once with PBS

It's a trade-off that requires careful consideration based on the specifics of your protocol
