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Moreover, these inactivated vaccines all use Alum as the adjuvant, which is generally a poor stimulator of T cell responses (Table 1)
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Nature 575 , 693698 (2019)
Briefly, the bacterial strains were grown to OD of 0.5 (mid-log phase) in regular 7H9 medium supplemented with OADS and 0.01% Tween 80
