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Stockwell, et al., "Ferroptosis: A Regulated Cell Death Nexus Linking Metabolism, Redox Biology, and Disease.", Cell , 2017 , 171 , 273
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The amplified fragment from SER3 was inserted into the AscI site of pATP406, pATP406- SHM2, pATP406- CYS4 , and pATP406- SHM2 / CYS4 to construct pATP406- SER3 , pATP406- SHM2 / SER3 , pATP406- CYS4 / SER3 , and pATP406- SHM2 / CYS4 / SER3 , respectively (Table 1)

Quantitative RT-PCR was used to measure the expression levels of miR-134-5p and CDCA3, while Western blot analysis assessed the levels of key regulatory proteins, including c-Myc, MMP9, CDCA3, and CDK1
