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These questions are experimentally tractable and would provide valuable data for researchers designing protocols with the modified compound
If the digestion remains incomplete at the highest enzyme-to-substrate ratios and longest time points, consider reengineering the protease cleavage site to include several glycines between the GST moiety and the target protein to decrease the likelihood of steric hindrance interfering with the cleavage (19, 20)
1997;273:E18591
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