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Similar content being viewed by others Introduction Vitamin B12 (B12), historically known as the anti-pernicious anemia factor by its ability to cure pernicious anemia, was first documented in 1926 1

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The crude peptides were subsequently purified by reverse-phase chromatography on a C18 column with a linear gradient of water and acetonitrile containing 0.1% TFA, and their molecular masses were verified by liquid chromatographymass spectrometry (LC/MS)
