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For HUVECs, endothelial growth medium-2 was supplemented with fetal bovine serum (FBS) (10 mL), hydrocortisone (2 mL), ascorbic acid (0.5 mL), human epidermal growth factor (hEGF-2) (0.5 mL), heparin (0.5 mL), human fibroblast growth factor-b (hFGF-) (2 mL), gentamicin sulfate-amphotericin (GA-1000) (0.5 mL), human recombinant insulin-like growth facto (VEGF) (0.5 mL) and R3-IGF (0.5 mL)