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The mitochondrial suspension was then centrifuged at 8500 g for 20 min at 4 C and the remaining pellet was resuspended in TRIzol reagent (15596018, Invitrogen) for downstream extraction of mitochondrial RNA using Direct-zol RNA miniprep (R2055, Zymo) with DNase treatment

[3] [6] [7] [8] Fatty acid synthesis occurs in the cytosol, where there is a high NADPH/NADP+ ratio available to drive the reactions forward

In animal models, MOTS-c treatment improved markers of mitochondrial health and reduced metabolic dysfunction associated with aging