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M., Fontella, F
Demuxed FAST5 were then base-called using Guppy 3.1.5 (Oxford Nanopore Technologies) with the model rna_r9.4.1_70bps_hac, and aligned to the human transcriptome from Ensembl , based on assembly GRCh38 ( supplemented with the CHIKV viral sequence using minimap2 80 v.2.17 with -uf -k14 -ax map-ont parameters (Supplementary Table 4)
The mixture was filtered and concentrated to obtain a crude product, which was purified by reversed-phase column chromatography to obtain 8 mg of off-white solid as compound 24 with a yield of 35%
doi: 10.1111/j.1600-079X.2009.00717.x 69 GoldmanBD
