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Methods Cell lines and reagents HEK293T and HeLa cells were maintained in high-glucose DMEM (Sigma-Aldrich, D6429) containing 10% fetal calf serum (Sigma-Aldrich, P4333), 100 U ml 1 penicillin and 100 g ml 1 streptomycin at 37 C and 5% CO 2
In breast cancer cells, the presence of Trimethylamine N-oxide (TMAO) can trigger GSDME-mediated pyroptosis (Wang H
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Beta Alanine: Delays muscular fatigue to enhance strength and power
