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ATP measurements The intracellular ATP concentrations of differentiated and differently treated mono- and co-cultured SH-SY5Y cells were measured using an ATP Determination Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA) according to the manufacturers protocol

J Korean Soc Food Sci Nutr

The Broader Implications of Glutathione and Milk Thistle Aging and Oxidative Stress As we age, our natural production of glutathione tends to decline, leading to various health challenges associated with oxidative stress

However, it is also possible to measure the GSSG concentration of the sample by first treating the sample with 4-vinylpyridine (4-VP) to sequester GSH