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One key consideration for preparing your glutathione agarose beads is how much volume of beads you need to use for your pull-down
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Result: In the presence of up to 5 mM TCEP, 10 mM DTT, or 50 mM -ME, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in absence of the respective reducing agents
