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Representative tissue specimens (i.e., the brain, liver, kidney, pancreas, stomach, small and large intestine, lungs, and heart taken at the end of the experiment, fixed in 10% neutral buffered formalin (pH 7.4) at room temperature for 24 h) were embedded in paraffin, sectioned at 4 m, and stained with hemalaun and eosin (H&E)

2.5.1.2 Large molecules analysis Regarding samples suspected of containing large peptides or proteins, the HPLC column was changed to a Supelco BIOshell A400 Protein C4 column (10 cm 2.1 mm

and age 85 or older, 35.8% (see Prevalence section)

In the application note Real-time monitoring of intracellular oxygen using MitoXpress-Intra kinetic measurements of intracellular oxygen were made on a BMG LABTECH microplate reader equipped with an Atmospheric Control Unit using time-resolved fluorescence and reagent injectors