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To limit glial scar formation, a cocktail of three mitotic inhibitors was added to the medium for the first 3 days of culturing: uridine, 5-fluorodeoxyuridine and cytosine--arabinofuranoside [4.4 mM each, Sigma, #U3003, #F0503 and #C1768]
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Stage 4: Clinical Damage At this stage, the haemoglobin levels begin to decrease, and there are clear indications of anaemia
