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PMID: 17260024

Cells were resuspended in annexin V binding buffer (BD Pharmingen) for staining and were analyzed using flow cytometry after 15 minutes of incubation at FL-1 and FL-3 channels

Based on the significant efficacy and acceptable toxicity profile, combination therapy with pembrolizumab and axitinib was approved by the FDA for treatment-nave patients with metastatic RCC

GSMe is usually a much weaker inhibitor in the HEDS assay or in assays with GSSR substrates 11,14,17 , suggesting that the competition between GSH and GSMe in the roGFP2 measurements occurred at the 1st and not the 2nd glutathione interaction site