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Our services include: Standard Pull-Down Assays Using tagged bait proteins immobilized on affinity beads (GST, His-tag, MBP), we capture interacting partners and analyze bound proteins via SDS-PAGE and Western blotting

Indeed, when GSAAT is reduced with gradually increasing amounts of DTT prior to incubation with NEM, either one Cys (Band 1, with 0.1 mM DTT) or no Cys residue of GSAAT is accessible for mPEG binding (with DTT 1 mM) ( Figure 2C )