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The T cells were rested overnight in complete culture medium (1640 medium containing 10% FBS and 1% penicillin-streptomycin) and then activated by stimulation with anti-CD3 (clone OKT3, eBioscience, USA) and anti-CD28 (clone CD28.2, eBioscience, USA) antibodies for 24 h to induce activation

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