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Insert the needle into the rubber stopper and push in the air into the vial, this creates a vacuum-like seal

Immunofluorescence The sections were occluded for 30 min with 5% BSA and incubated with anti-SLC7A11, anti-GPX4, anti-ACSL4, anti-CD31, anti-Ecadherin, and anti-SMA primary antibodies for 2 h, followed by incubation at 26 C with specific secondary antibodies

Fei N, Bruneau A, Zhang X, et al

Unlike nasal sprays, which often suffer from inconsistent deposition due to incorrect device orientation and patient technique, nasal nebulisers generate aerosol particles capable of penetrating the nasal cavity regardless of the device's positioning or inhalation angle