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Subsequently, according to the method described in the previous study ( 2.7 Immunofluorescence (IF) After transcardial perfusion in mice, brain tissues were harvested and pretreated with gradient dehydration in 20% and 30% sucrose solutions at 4C (to reduce ice crystal damage), then embedded in optimal cutting temperature compound and sectioned for 20 m thickness using cryostat

The results demonstrated that the first and the second protonation constants were predominated by the overlapping protonation of the amino and the thiolate site, the amino being typically more favored

This is practical when the peptides are compatible and the combined volume fits the sublingual space

For these reasons, it is often more difficult to diagnose in women