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Plates were preloaded with 80 nl of 10 mM compounds using a Mosquito LV pipetting system (SPT Labtech)
[ 135 ] In addition, the knockdown of BACH1 also suppresses the production of ROS and NAD(P)H oxidase subunit NOX2 expression and decreases cell migration in VSMCs from spontaneously hypertensive rats (SHRs)
They also validated the results using male Sprague Dawley rats
This has additional consequences for hepatic function, including bile synthesis and release [72], but also regulates anabolic processes, such as glycogen, lipid, and protein synthesis [61]
