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Crude peptides were first purified by reverse phase HPLC using a CI 8 column using gradient elution
& Happel, L
Water balance is maintained by various regulatory mechanisms

Effects of Bisphosphonates on Alkaline Phosphatase Activity, Mineralization, and Prostaglandin E2 Synthesis in the Clonal Osteoblast-like Cell Line MC3T3-E1
