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Description
Although the heavy subunit by itself can catalyze the formation of L--glutamyl-L-cysteine, binding with the modifier subunit enhances the enzyme activity by lowering the Km for glutamate and ATP, and increasing the Ki for GSH inhibition

Compounding this is the regulatory gap

Flash column chromatography was carried out using glass chromatography columns packed with 4063 m silica gel or with the Biotage Selekt high-performance automated flash purification system

Percival, Immunomodulatory Effects of Glutathione, Garlic Derivatives, and Hydrogen Sulfide
