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Plasma samples were diluted with three volumes of ethanol containing 1% formic acid, and the resultant supernatant was subjected to online sample clean-up by turbulent flow chromatography on a Cyclone (50 0.5 mm) column from Thermo Fisher prior to LCMS analysis

To prepare Shepu Appu, mix one sachet with 250 ml of water

It was also shown that the multifunctional nanocomponent could cross the BBB after intravenous injection in the tail of mice[138]

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