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Thus, many signaling and metabolic pathways in brain tissues provoke enhanced ROS formation, which is often exacerbated by the region-specific sensitivity of the brain to OS

The samples were stained with primary antibody, mouse monoclonal anti-keratin 20 (Abcam, ab854, 1:200) and secondary antibody, Alexa Fluor 647 donkey anti-mouse IgG (Invitrogen, A-31571, 1:1,000), then further stained with Alexa Fluor 555 Phalloidin (ThermoFisher, A34055, 1:200) and 4,6-diamidino-2-phenylindole (DAPI) (ThermoFisher, D1306, 1:1,000)

Mulholland et al., 1991