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All primary antibody incubations (1:1000 in TBST, overnight at 4 C) were followed by incubation with a secondary HRP-conjugated antibody (1:5000 in TBST, 1 h at room temperature), and the signals were visualized using a chemiluminescent HRP substrate on a chemiluminescence imaging system (CHAMPCHEMI TM Chemiluminescent Imaging and Analysis System)

Bai Y, Meng L, Han L, Jia Y, Zhao Y, Gao H, et al

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Over-expression of aromatase in male transgenics causes increased mammary growth and histological changes similar to gynecomastia, increased estrogen and progesterone receptors, and increased downstream breast growth factors such as TGF-beta and FGF (17)