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The development of biomarkers faces considerable challenges

A deficiency in catalytically active GPX4 impairs the cellular reductive capacity, which results in uncontrolled intracellular accumulation of lipid hydroperoxides (Dixon and Stockwell 2019)
In whole-cell current clamp experiments, hyperpolarizing or depolarizing current pulses (500 ms, from 10 to 30 pA in 5-pA steps) were injected into neurons to determine input resistances or trigger action potentials

The retention of sulfur from SAA or from the dietary supplements administered during the ingestion of low or marginal levels of protein as compared to the enhanced excretion during higher levels of intake provided valuable clues (Fig
