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Approaches for investigating p K a values of individual Cys residues in proteins often require the use of other methods, as well, including spectroscopic detection of small differences between absorbances of thiolate and thiol around 240 nm [37, 38], NMR detection of shifting protonation state relative to solution pH [39], readouts associated with chemical reactivity [40, 41], or functional assays of the thiol/thiolate group of interest [42, 43]
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Gerontology 1994
