Glutathione Disulfide - an overview
US$ 21.34
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Description
Preparation of protein lysates and immunoblot analysis Cell lysates were collected by scraping into RIPA buffer (PBS, 1% IGEPAL CA-630 (I3021, Sigma), 0.5% sodium deoxycholate (D6750, Sigma-Aldrich), 0.1% sodium dodecyl sulfate (SDS) (BP166, ThermoFisher Scientific)) containing 1 mM AEBSF (328110500, ThermoFisher Scientific), 1 x protease inhibitor cocktail (11836153001, Roche) and 1x Halt phosphatase inhibitor cocktail (78420, ThermoFisher Scientific)
Additionally, the BV bacteria produce high levels of acetate and succinate that are suggested to increase TLR4 expression leading to production of pro-inflammatory cytokines [48, 53, 64]
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June 24, 2017
