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Methodologically rigorous studies that couple pathway inhibition to predefined immune and clinical endpoints, deploy standardized biospecimen workflows and use harmonized safety reporting are required to determine whether neurotrophin-targeted strategies can remodel the osteosarcoma microenvironment toward durable immune control

Abbreviations COX-2: Cyclooxygenase-2 5-LOX: 5-Lypoxygenase LC 50 : Median lethal dose MIC: Minimum inhibitory concentration TLC: Thin layer chromatography ppm: Parts per million CDCl 3 : Deuterated chloroform R f : Retardation factor m: Multiplet d: Doublet dd: Doublet of doublet ddd: Doublet of doublet of doublet s: Singlet References Mizushima, Y

Compared with the IDD group, IDD mice injected with hADSCs showed increased disc height index, MRI index and mean T2 intensities, as well as the attenuated histologic grading of the annulus fibrosus (AF) and NP accompanied by the upregulation of GAG and COL2