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Description
To achieve adipocyte differentiation, 3T3-L1 pre-adipocytes were incubated with differentiation medium (DMEM (1X) + GlutaMAX with 10% fetal bovine serum (FBS) and 1 10 5 U/L penicillin and streptomycin, containing insulin 10 6 M, dexamethasone 10 7 M and 3-isobutyl-1-methylxanthine 10 3 M to starting adipogenesis (day 0)

Patients expect to see improvements within days, with full effects typically visible two to four weeks post-treatment

Individual responses vary based on genetics, baseline health status, adherence to protocol, and lifestyle factors

PubMed Central , Human Molecular Genetics , vol
