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Louis, MO), The lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA total protein assay, and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten minutes at 95 C

Cilia, F

Instead, we utilised the high nucleophilic reactivity of RSS and RS(S) n S (due to their low pK a and the -effect of the non-terminal sulfur atom) that enables reaction with iodoacetamide (IAM) to generate reaction products that incorporate the terminal sulfane sulfur (Fig
