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Finally, the Ni-NTA resin-bound proteins were eluted with BF + 200 mM imidazole, fractionated on a reducing 12% SDS-PA gel and probed with TRX f1 and NTRC antibodies following immunoblotting

Pathways regulating ferroptosis in AD Kelch-like ECH-associated protein 1 (Keap1)/Nrf2)/antioxidant response element (ARE) pathway In cellular defense against oxidative stress, the Keap1/Nrf2/ARE signaling pathway regulates the expression of various proteins involved in detoxification and antioxidant defense, positioning it as a potential target for AD treatment (61)