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Obese patients on contrave also demonstrated significant improvements in important markers of cardiometabolic risk, including waist circumference, high-density lipoprotein cholesterol, insulin resistance and triglycerides

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Proteins were transferred from the gel into a nitrocellulose membrane (Amersham, UK) to perform the western blotting by applying the Semi-Dry-Blotting system (Bio-Rad, USA) for 45 min at 25 V
