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cDNA was amplified in MicroAmp Optical 96-well reaction plates with SYBR Green PCR Master Mix (Applied Biosystems) on an Applied Biosystems Prism 7000 sequence detection system
Unwinding of the 3X tail by HCV NS3h is a tempting model, and our data provide a hint towards distinct functions of viral helicases in the initiation of negative-strand synthesis
Senomorphics are currently under development to lower this inflammatory burden
