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Description
Analysis of PolyQ aggregation Thirty animals containing PolyQ repeat length polymorphisms, AM140 ( rmIs132 [P unc-54 ::Q35::YFP]) and AM141 ( rmIs133 [P unc-54 ::Q40::YFP]), were scored for the actual number of YFP puncta aggregates present throughout the animal
Following staining, cells were washed five times with pre-warmed DMEM/F12, then transferred to ice, washed three times with 1 PBS, and fixed with 4% paraformaldehyde for 30 min on ice in the dark
Diindolylmethane (DIM) Diindolylmethane is a compound formed in the body from plant substances of cruciferous vegetables such as cabbage, Brussels sprouts, cauliflower, and broccoli
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