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The ratio of Firmicutes to Bacteroidetes is a commonly-studied change with an increase often linked to HFD consumption/obesity in mice 43,44 and humans 45,46 (for review, see John and Mullin, 2016 47 )

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Taxonomic summaries and alpha (observed) and beta (weighted or unweighted UniFrac distances) diversity metrics and taxonomic classifications were computed using QIIME software 32 and downstream analyses were done with in-house Perl and R scripts

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