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Metabolomic profiling and stable isotope labeling To quantify metabolites, each cell line was seeded 200,000 cells/well in triplicate (n=3) in 6-well plates with media as described in the cell culture methodology section until achieving approximately 80% confluency (approximately 24 hours)

Analysis 82, 103226

Pregnant women with elevated homocysteine levels have an increased risk of gestational diabetes, miscarriage, preeclampsia, and premature delivery.29 Additionally, infants born to women with folate and vitamin B12 deficiencies and elevated blood homocysteine levels were found to have an increased risk of developing insulin resistance by age 6, compared with women with values in the normal range.2 Daily supplementation with 5 to 10 mg of folic acid for up to 12 weeks significantly lowered plasma homocysteine levels among 426 patients with type 2 diabetes