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During neuropathy, the numbers of ionized calcium binding adapter molecule 1 (Iba1) (microglia) and glial fibrillary acidic protein (GFAP) (astrocyte) immune-positive cells showed an increase in the dorsal horn of the spinal cord, concomitantly with a decrease of pain threshold and a glia density increase in various supraspinal sites [88]

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The POI is applied as a mixed solution with various contaminants typically from a cell extract or culture supernatant in the buffered aqueous mobile phase whereby it forms a non-covalent complex with the immobilized ligand